Simultaneous Analysis of Chlorogenic Acid, Epicatechin Gallate, and Rosmarinic Acid from the LOL (Ligularia stenofelphala, Orostachts japonica, Lavandula anfustifola) Cosmetic Composite and its Antioxidative Activity

Purpose: As the demand for natural cosmetics increases, properly managing production and quality control should be prioritized. Chlorogenic acid (CGA), epicatechin gallate (ECG), rosmarinic (RMA) were selected to validate cosmetic composite LOL, a mixture of 50% EtOH extract Ligularia stenocephala (LS), Orostachys japonica (OJ), Lavandula angustifolia (LA). Methods: Highperformance liquid chromatography with diode array detector (HPLC-DAD) was used simultaneously analyze three LOL compounds from (Ligularia , ). Linearity, accuracy, precision analyses performed using HPLC-DAD, anti-oxidative effects evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Results: All extracts showed high DPPH effect, detected marker significant linearity (R2≥0.9997). The limit detection (LOD) CGA, ECG, RMA 0.83 μg/mL, 0.33 0.20 respectively. Meanwhile, quantification (LOQ) 2.51 0.99 0.61 Method validation acceptable (intra-and inter-day precision, RMA, 1.77, 1.42, 0.80% 1.09, 0.72, 1.42%, respectively) recovery (CGA, 102.00%–117.78%, 94.40%-108.06%, 93.79%–107.05%). Conclusion: contents markers 13.99 μg/mL 8.28 8.40 This study also suggests that developed method can composition process.